Cloning and expression of a 12 kDa serospecific epitope of Wuchereria bancrofti.
Identifieur interne : 008019 ( Main/Exploration ); précédent : 008018; suivant : 008020Cloning and expression of a 12 kDa serospecific epitope of Wuchereria bancrofti.
Auteurs : Pankaj Dabir [Inde] ; Snehal Dabir ; M V R. ReddySource :
- Immunology and cell biology [ 0818-9641 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Antigènes d'helminthe (biosynthèse), Antigènes d'helminthe (génétique), Antigènes d'helminthe (immunologie), Antigènes d'helminthe (métabolisme), Biotine (génétique), Biotine (immunologie), Biotine (métabolisme), Clonage moléculaire, Données de séquences moléculaires, Filariose lymphatique (diagnostic), Filariose lymphatique (immunologie), Filariose lymphatique (sang), Humains, Protéines d'helminthes (génétique), Protéines d'helminthes (immunologie), Protéines d'helminthes (métabolisme), Protéines de fusion recombinantes (génétique), Protéines de fusion recombinantes (immunologie), Protéines de fusion recombinantes (métabolisme), Wuchereria bancrofti (génétique), Wuchereria bancrofti (immunologie), Wuchereria bancrofti (métabolisme), Épitopes immunodominants (génétique), Épitopes immunodominants (immunologie).
- MESH :
- biosynthèse : Antigènes d'helminthe.
- diagnostic : Filariose lymphatique.
- génétique : Antigènes d'helminthe, Biotine, Protéines d'helminthes, Protéines de fusion recombinantes, Wuchereria bancrofti, Épitopes immunodominants.
- immunologie : Antigènes d'helminthe, Biotine, Filariose lymphatique, Protéines d'helminthes, Protéines de fusion recombinantes, Wuchereria bancrofti, Épitopes immunodominants.
- métabolisme : Antigènes d'helminthe, Biotine, Protéines d'helminthes, Protéines de fusion recombinantes, Wuchereria bancrofti.
- sang : Filariose lymphatique.
- Animaux, Clonage moléculaire, Données de séquences moléculaires, Humains.
English descriptors
- KwdEn :
- Animals, Antigens, Helminth (biosynthesis), Antigens, Helminth (genetics), Antigens, Helminth (immunology), Antigens, Helminth (metabolism), Biotin (genetics), Biotin (immunology), Biotin (metabolism), Cloning, Molecular, Elephantiasis, Filarial (blood), Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (immunology), Helminth Proteins (genetics), Helminth Proteins (immunology), Helminth Proteins (metabolism), Humans, Immunodominant Epitopes (genetics), Immunodominant Epitopes (immunology), Molecular Sequence Data, Recombinant Fusion Proteins (genetics), Recombinant Fusion Proteins (immunology), Recombinant Fusion Proteins (metabolism), Wuchereria bancrofti (genetics), Wuchereria bancrofti (immunology), Wuchereria bancrofti (metabolism).
- MESH :
- chemical , biosynthesis : Antigens, Helminth.
- chemical , genetics : Antigens, Helminth, Biotin, Helminth Proteins, Immunodominant Epitopes, Recombinant Fusion Proteins.
- chemical , immunology : Antigens, Helminth, Biotin, Helminth Proteins, Immunodominant Epitopes, Recombinant Fusion Proteins.
- chemical , metabolism : Antigens, Helminth, Biotin, Helminth Proteins, Recombinant Fusion Proteins.
- blood : Elephantiasis, Filarial.
- diagnosis : Elephantiasis, Filarial.
- genetics : Wuchereria bancrofti.
- immunology : Elephantiasis, Filarial, Wuchereria bancrofti.
- metabolism : Wuchereria bancrofti.
- Animals, Cloning, Molecular, Humans, Molecular Sequence Data.
Abstract
The immunoscreening of a microfilarial cDNA library of Wuchereria bancrofti with microfilaraemic sera revealed many positive clones expressing filarial antigens. One immunoreactive clone, designated PMR1, was shown to encode a protein of 114 amino acid residues. The cDNA fragment was subcloned into an expression vector, Pinpoint XaT. The resulting recombinant (r)PMR1-biotin fusion protein was expressed in Escherichia coli (BL21 [DE3] pLys) and was affinity purified on avidin resin. Analysis of sera of different groups for filarial antibodies against rPMR1 showed it to be highly reactive with microfilaraemic and clinical filarial sera compared to its reactivity with endemic and nonendemic controls. This indicates that the gene sequence of cDNA is expressing an immunodominant epitope, which could be useful in serodiagnosis of lymphatic filariasis.
DOI: 10.1111/j.1440-1711.2005.01359.x
PubMed: 16174102
Affiliations:
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Le document en format XML
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(immunology)</term><term>Molecular Sequence Data</term><term>Recombinant Fusion Proteins (genetics)</term><term>Recombinant Fusion Proteins (immunology)</term><term>Recombinant Fusion Proteins (metabolism)</term><term>Wuchereria bancrofti (genetics)</term><term>Wuchereria bancrofti (immunology)</term><term>Wuchereria bancrofti (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Antigènes d'helminthe (biosynthèse)</term><term>Antigènes d'helminthe (génétique)</term><term>Antigènes d'helminthe (immunologie)</term><term>Antigènes d'helminthe (métabolisme)</term><term>Biotine (génétique)</term><term>Biotine (immunologie)</term><term>Biotine (métabolisme)</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Filariose lymphatique (diagnostic)</term><term>Filariose lymphatique (immunologie)</term><term>Filariose lymphatique (sang)</term><term>Humains</term><term>Protéines d'helminthes 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bancrofti</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cloning, Molecular</term><term>Humans</term><term>Molecular Sequence Data</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Humains</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The immunoscreening of a microfilarial cDNA library of Wuchereria bancrofti with microfilaraemic sera revealed many positive clones expressing filarial antigens. One immunoreactive clone, designated PMR1, was shown to encode a protein of 114 amino acid residues. The cDNA fragment was subcloned into an expression vector, Pinpoint XaT. The resulting recombinant (r)PMR1-biotin fusion protein was expressed in Escherichia coli (BL21 [DE3] pLys) and was affinity purified on avidin resin. Analysis of sera of different groups for filarial antibodies against rPMR1 showed it to be highly reactive with microfilaraemic and clinical filarial sera compared to its reactivity with endemic and nonendemic controls. This indicates that the gene sequence of cDNA is expressing an immunodominant epitope, which could be useful in serodiagnosis of lymphatic filariasis.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Dabir, Snehal" sort="Dabir, Snehal" uniqKey="Dabir S" first="Snehal" last="Dabir">Snehal Dabir</name><name sortKey="Reddy, M V R" sort="Reddy, M V R" uniqKey="Reddy M" first="M V R" last="Reddy">M V R. Reddy</name></noCountry><country name="Inde"><noRegion><name sortKey="Dabir, Pankaj" sort="Dabir, Pankaj" uniqKey="Dabir P" first="Pankaj" last="Dabir">Pankaj Dabir</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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